Journal: PLoS Pathogens

Article Title: Phosphodiesterase-4 Inhibition Alters Gene Expression and Improves Isoniazid – Mediated Clearance of Mycobacterium tuberculosis in Rabbit Lungs

doi: 10.1371/journal.ppat.1002262

Figure Lengend Snippet: (A) Level of INH detected in the plasma of rabbits treated with a bolus dose of either 25 or 50 mg/kg of INH by gavage. INH levels were estimated by HPLC-mass spectrometry as explained in the section (B) Effect of different modes of administration of INH on rabbit plasma INH levels. Rabbits were treated with INH either by gavage using a rubber feeding tube, or in drinking water. Plasma INH from treated rabbits was separated from the blood collected at indicated time points, post-administration, to measure the INH concentration by HPLC-mass spectrometry as explained in the Methods section. The horizontal dotted line in (B) represents the optimal level of quantification of INH.

Article Snippet: The concentration of INH in the plasma of Mtb infected and treated rabbits were measured using Sciex API4000 (Applied Biosystems, CA, USA) mass spectrometer coupled to Symbiosis Pharma HPLC system (Spark Holland B.V, The Netherlands).

Techniques: Clinical Proteomics, Mass Spectrometry, Concentration Assay